Lazaroid U74006F for canine small bowel preservation.

Adults mongrel dogs, weighing from 18 to 24 kg, were used. The entire small bowel was procured and flushed with cold lactated Ringer's solution containing heparin through the superior mesenteric artery. The intestinal lumen was also irrigated with the same solution. After the graft was preserved in the cold lactated Ringer's ,olution for 24 hours, it was transplanted orthotopically into the ,;ame animal. The U74006F was intravenously administered at a dose of 0 mg/kg (Laz(O», 5 mg/kg (Laz(5», 10 mglkg (Laz(10», or 15 mg/kg (Laz( 15» 30 minutes before graft removal and 30 minutes before graft reperfusion. Five animals were randomly tested at each group. For histological examination and biochemical determinations including maltase, malondialdehyde (MDA), myeloperoxidase (MPO), phospholipids (PL), and energy charge (EC), short segments of the ileum were collected before procure-